| Product number | 75/51-0010 |
| Status | In stock |
| Size | 1 mg |
| Price | EUR 102 |
| Shortname | A(-)-PSM |
| Synonym | A- -Porcine Submaxillary Mucin (PSM- |
| Storage | -18 °C |
Source: Porcine submaxillary glands
Isolated from glands that are typed for their blood group A+ or A-phenotype by standard serological methods. The mucins are prepared as described in ref 1. The mucins have been treated with 0.1 M trifluoroacetic acid at 100 °C for 1h, which removes all sialic acid and most of the fucose. Composition: Information on the structures of the carbohydrate chains on A+ and A-mucins are given in ref 2.
Use: Use the mucins as acceptors for the following glycosyltransferases: CMP-Neu5Ac:Galb1-3GalNAca-R/a(2-3)-sialyltransferase (EC 2.4.99.4)
UDP-GlcNAc:Galb1-3GalNAca-R/b(1-6)-N-acetylglucosaminyltransferase (EC 2.4.1.102)
GDP-Fuc:Galb1-3GalNAca-R/a(1-2)-fucosyltransferase (EC 2.4.1.69)
Add water or buffer and allow to dissolve over night.
Storage: 0-5 °C
References